Kdo determination

Full list of chemicals

1. Start with hydrolysis with 0.1 M TFA for 80 °C for 1 h.
2. Reduce with NaBH₄
3. Continue with hydrolysis with 2 M TFA for 100 °C for 2 h
4. As usual

• 0.5 mL of 20 mM buffer will add approximately 1 mg of salt in your sample.