Table of Contents
Removal of phospholipids from LPS
Introduction
Reagents
Procedure
- Check sample for the presence of C16 and C18 fatty acids by GLC.
- If phospholipds are present ( C16 and C18 fatty acids) the extract with CHCl3:CH3OH:H2O 16:8:1
- Verify the absence of phospholipds by TLC (CHCl3:CH3OH:H2O:Triethylamine 30:15:2.5:0.1).
Comments
References
- J. Bact. 175 (1993) 2988-2993
- Carbohydr. Res. 175 (1988) 273-282