Removal of phospholipids from LPS

Introduction

Reagents

Procedure

  1. Check sample for the presence of C16 and C18 fatty acids by GLC.
  2. If phospholipds are present ( C16 and C18 fatty acids) the extract with CHCl3:CH3OH:H2O 16:8:1
  3. Verify the absence of phospholipds by TLC (CHCl3:CH3OH:H2O:Triethylamine 30:15:2.5:0.1).

Comments

References

  • J. Bact. 175 (1993) 2988-2993
  • Carbohydr. Res. 175 (1988) 273-282
 
sop/sop307.txt · Last modified: 2010/10/28 14:56 by magnusl