====== Kdo determination ======

===== Introduction =====

==== Reagents ====

[[chemicals|Full list of chemicals]]

==== Procedure ====

  - Start with hydrolysis with 0.1 M TFA for 80 °C for 1 h.
  - Reduce with NaBH<sub>4</sub>
  - Continue with hydrolysis with 2 M TFA for 100 °C for 2 h
  - As usual

==== Comments ====

  * 0.5 mL of 20 mM buffer will add approximately 1 mg of salt in your sample.