Table of Contents

Removal of phospholipids from LPS

Introduction

Reagents

Full list of chemicals

Procedure

  1. Check sample for the presence of C16 and C18 fatty acids by GLC.
  2. If phospholipds are present ( C16 and C18 fatty acids) the extract with CHCl3:CH3OH:H2O 16:8:1
  3. Verify the absence of phospholipds by TLC (CHCl3:CH3OH:H2O:Triethylamine 30:15:2.5:0.1).

Comments

References